In situ hybridization in Actinidia using repeat DNA and genomic probes |
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| Authors: | G. Yan R. G. Atkinson A. R. Ferguson M. A. McNeilage B. G. Murray |
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| Affiliation: | (1) School of Biological Sciences, The University of Auckland, Private Bag 92 019, Auckland, New Zealand, NZ;(2) Horticulture and Food Research Institute of New Zealand, Private Bag 92 169, Auckland, New Zealand, NZ |
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| Abstract: | In situ hybridization has been used to probe chromosome spreads of hexaploid Actinidia deliciosa (kiwifruit; 2n=6x=174) and tetraploid A. chinensis (2n=4x=116). When a species-specific repeat sequence, pKIWI516, was used, six hybridization sites were observed in some accessions of tetraploid A. chinensis and all of A. deliciosa. Southern analysis with the pKIWI516 probe revealed that there are two types of tetraploid A. chinensis. Genomic probes from diploid A. chinensis (2n=2x=58) did not differentiate the genomes of hexaploid A. deliciosa and tetraploid A. chinensis, irrespective of the presence or absence of blocking DNA. The results indicate that the genomes of polyploid Actinidia species are similar but not identical. The origin of A. deliciosa is discussed. Received: 29 June 1996 / Accepted: 5 July 1996 |
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| Keywords: | Actinidia Kiwifruit Repeat sequence In situ hybridization |
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