Yeast biodiversity in the guts of several pests on maize; comparison of three methods: classical isolation,cloning and DGGE |
| |
Authors: | Orsolya Molnár Michael Wuczkowski Hansjörg Prillinger |
| |
Institution: | (1) Austrian Center of Biological Resources and Applied Mycology (ACBR), Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria |
| |
Abstract: | The yeast biodiversity in the guts of several pests (Diabrotica virgifera, Helicoverpa armigera, Ostrinia nubilalis) on maize from two isolation sources was assessed by cultivation-dependent and cultivation-independent methods. These yeasts
are considered to bear a potentially high biotechnological relevance due to their potential ability to degrade several mycotoxins
incorporated by their hosts. The 97 isolated yeast strains showed 21 different partial sequence types of the 26S rRNA gene
which could be assigned to 10 different genera. The determined genera and species are discussed in terms of the meaning of
their taxonomic status or their occurrence in nature. Two cultivation-independent methods, cloning and DGGE, were compared.
We propose the combination of these methods as well as the combination of both cultivation-independent and cultivation-dependent
approaches, for gaining better insights into fungal biodiversity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|