东亚飞蝗山西两地理种群酯酶特性的比较研究

对东亚飞蝗山西临猗和永济2个地理种群的酯酶特性进行了比较研究.非变性聚丙烯酰胺凝胶电泳图谱显示:以a-乙酸萘酯为底物染色,2个东亚飞蝗种群谱带差别不明显.但是,酯酶动力学研究结果表明:以a-乙酸萘酯和α-丁酸萘酯为底物时,永济种群的酯酶活性分别是临猗种群的1.81倍和1.20倍.永济种群酯酶活性的增高可能与非变性聚丙烯酰胺凝胶电泳图谱显示出较临猗种群多出的酶带有关.体外酯酶抑制动力学研究表明:永济和临猗2种群所含酯酶大都为B型酯酶,其含量分别为84.94%和91.47%.永济种群对对氧磷的耐受性要高于临猗种群,我们推测可能与2种群马拉硫磷使用背景不同有关.

Characterization and comparison of general esterases from two field populations of the oriental migratory locust, Locusta migratoria manilensis ( Meyen ) (Orthoptera: Acrididae) from Shanxi Province

General esterases in two field populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen),respectively from Yongji and Linyi, Shanxi Province, were characterized and compared. No distinct difference was detected in banding patterns of general esterases between the two populations, when α-naphthyl acetate was used as substrate in nondenaturing polyacrylamide gel electrophoresis ( PAGE). Nevertheless, the general esterase activities in the Yongji population were 1.81- and 1.20-fold higher, respectively, than those of the Linyi population when α-naphthyl acetate and α-naphthyl butyrate were used as substrates. Increased esterase activity in the Yongji population might be correlated to several additional esterase bands detected with non-denaturing PAGE. Inhibition studies showed that 84.94% and 91.47 % of esterases were Btype in Yongji and Linyi population, respectively. The Linyi population appeared to be more susceptible to malaoxon than the Yongji population. We estimated that such differences might be attributed to the different control practices for locusts in the two areas.