A simple and cost-effective spectrophotometric assay for cephalosporin C usingAlcaligenes faecalis |
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Authors: | Yong-Ho Khang Hariharan Shankar Fred Senatore |
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Institution: | (1) Department of Chemical Engineering, Texas Tech University, 79409 Lubbock, Texas, USA |
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Abstract: | Summary A spectrophotometric assay for cephalosporin C was established usingAlcaligenes faecalis as a test organism. The optimal pH range for the sensitivity of the assay was pH 6.7–7.4. The dose-response curve became linear after 4 h incubation with 15% (v/v) dosage. Deviations between spectrophotometric and chemical assays were within 6%. The cost of the spectrophotometric assay is about one-hundredth that of the chemical assay. |
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