Neutralizing effect of zinc oxide on dehydroabietic acid-induced toxicity on human polymorphonuclear leukocytes |
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Authors: | B. Sunzel T. A. Söderberg C. -O. Reuterving G. Hallmans S. E. Holm L. Hänström |
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Affiliation: | 1. Department of Oral Surgery, University of Ume?, Ume?, Sweden 2. Department of Hand Surgery, University of Ume?, Ume?, Sweden 3. Department of Pathology and Nutritional Research, University of Ume?, Ume?, Sweden 4. Department of Clinical Bacteriology, University of Ume?, Ume?, Sweden 5. Department of Periodontology, University of Ume?, Ume?, Sweden
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Abstract: | The cytotoxic effect of dehydroabietic acid (DHAA), a resin acid found in rosin, was studied on human polymorphonuclear leukocytes using leakage of 51Cr from prelabeled cells, supravital staining, and transmission electron microscopy. DHAA caused a strong dose-related release of 51Cr, a high uptake of trypan blue, and total cell necrosis, as seen in transmission electron microscopy. Albumin slightly reduced the toxic effects, whereas the addition of zinc in various forms strongly inhibited these toxic effects of DHAA in the concentration range of 10-500 micrograms/mL. In the presence of albumin, zinc oxide as a suspension inhibited the damage of the cell membranes more than a filtrate of zinc oxide, indicating a subsequent slow release of zinc from the zinc oxide. |
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Keywords: | KeywordHeading" >Index Entries Rosin dehydroabietic acid polymorphonuclear leukocytes zinc oxide albumin cytotoxicity 51Cr-release method |
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