Abstract: | The effect of Brilliant Green on motility was studied with Salmonella anatum, S. derby, S. tennessee, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. Semisolid tryptic soy-agars containing 0, 20, or 40 mg of Brilliant Green per liter were used as the motility media. Both concentrations of Brilliant Green inhibited the growth of the non-Salmonella species through the semisolid agar. For 24 hr, the Brilliant Green appeared to limit the growth of the salmonellae; however, by 48 hr the salmonellae were able to grow through the semisolid agars. The presence of Brilliant Green in the motility media aided in the detection of Salmonella when mixed cultures were used. |