Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon |
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Authors: | Bernard A Konfortov Victoria E Licence J Ross Miller |
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Institution: | (1) Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK, GB |
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Abstract: | In order to assess the extent of DNA sequence variation in cattle, introns and exons from both the leptin and Amyloid Precursor
Protein (APP) genes have been sequenced in a panel of DNAs derived from 22 diverse animals. Direct DNA sequencing of PCR products
was used; thus, 44 chromosomes were studied. Polymorphisms were identified by manual scanning of sequence chromatograms and
computerized sequence analysis. Twenty Single Nucleotide Polymorphisms (SNPs) were detected in 1788 bp sequenced from the
leptin gene, giving a frequency of 1 SNP per 89 bp. Twenty-four SNPs were detected in a 458-bp fragment of the APP gene; 23
of the polymorphisms were contained in a 302-bp intron 16 fragment. This equates to an SNP frequency of 1 per 13 bp for the
intron. We can thus conclude that this portion of the bovine APP gene constitutes a hypermutable region. Nucleotide sequence
diversity values of 0.019 and 0.0026 were obtained for APP and leptin respectively.
Received: 22 April 1999 / Accepted: 25 July 1999 |
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