Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro |
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| Authors: | Maurice P De Proft Ludo J Maene Pierre C Debergh |
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| Institution: | Centrum voor de Hormonale Groeiregulatie bij Ptanten, Univ. Instelling Antwerpen, Dept Biologie, Universiteitsplein 1, B-2610 Wil-rijk, Belgium;Centrum voor de Studie van de Voortplanting bij Tuin-bouwgewassen, I. W. O. N. L., Coupure links 653, B-9000 Gent, Belgium;Lab. for Horticultural Sciences, State Univ. Gent, Coupure links 653, B-9000 Gent, Belgium. |
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| Abstract: | Subcultured explants of Magnolia soulangeana Soul, were incubated in tissue culture containers fitted with different types of closures. Type of closure affected the CO2 concentration, with levels as high as 14% CO2 being detected. The ethylene concentration increased gradually with time, to as much as 2–3 ppm after 9 weeks. There was a large variation in the composition of the atmosphere within the containers of any one type. The way by which a container was closed influenced exchange of the inner gas phase with the surrounding atmosphere and was important in determining the development of the cultured tissues. |
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| Keywords: | Chlorophyll culture vessel micropropagation |
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