Translational signals of a major head protein gene of bacteriophage lambda |
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Authors: | Mordechai Suissa Shoshy Altuvia Simi Koby Hilla Giladi and Amos B Oppenheim |
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Institution: | (1) Department of Molecular Genetics, The Hebrew University-Hadassah Medical School, Jerusalem, Israel;(2) Present address: BioLum Ltd., P.O.B. 9231, Technion City, Haifa, Israel |
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Abstract: | Summary The D gene of bacteriophage which codes for a major head protein is expressed at a high level during growth. We have constructed a set of D-lacZ gene fusions in order to examine the factors determining the high efficiency of the D translational initiation signals. It was found that an integral sequence, 300 bp long and upstream of the ATG initiation codon, is required for maximal protein synthesis. |
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Keywords: | Translation initiation Bacteriophage lambda |
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