Oxidations by microbial NADH plus FMN-dependent luciferases from Photobacterium phosphoreum and Vibrio fischeri |
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Affiliation: | 1. Groningen Research Institute of Pharmacy, Chemical and Pharmaceutical Biology, Groningen, The Netherlands;2. University of Greifswald, Institute of Biochemistry, Greifswald, Germany |
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Abstract: | The NADH plus FMN-dependent luciferase from Photobacterium phosphoreum NCIMB 844 has been shown to act as a Baeyer-Villiger monooxygenase able to perform regio-, and where relevant, enantioselective biotransformations of various xenobiotic aliphatic and alicyclic ketones by nucleophilic oxygenation. The useful lactone (−)-(1S,5R)-2-oxabicyclo [3.3.0]oct-6-en-3-one was produced with high optical purity (> 95% ee). A similar biotransformation was recorded with the equivalent luciferase from Vibrio fischeri ATCC 7744. |
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