Pseudogymnoascus roseus的生物学特性及液体发酵培养基的筛选

分离自冬虫夏草子座的子囊菌, 产生大量具网状包被的子囊果。其代表菌株Pseu-F经形态学及分子鉴定, 确定为Pseudogymnoascus roseus。该菌株的适宜生长温度为17.5 °C?20.0 °C; 用正交试验法对该菌株进行了液体发酵培养基筛选试验, 试验因素包括马铃薯、黄豆、蔗糖+葡萄糖、蛋白胨、酵母膏、矿物盐、维生素等, 筛选出的优化液体发酵培养基为(g/L): 蔗糖20+葡萄糖10, 蛋白胨10, 酵母膏5, 黄豆50, 马铃薯100。

Morphological characteristics and submerged culture medium screening of Pseudogymnoascus roseus

Isolates from the stroma of Ophiocordyceps sinensis, produced generously ascocarps with reticuloperidium. Morphological characteristics and molecular identification indicated that the repre-sentative strain Pseu-F was Pseudogymnoascus roseus Raillo. The optimum temperatures for mycelial growing were 17.5 °C?20.0 °C. Submerged culture media were screened by means of orthogonal design experiments. Test factors included sucrose, glucose, peptone, yeast extract, potato, soybean, mineral salt and Vitamins. Based on the results of tests, the optimized combinations of culture medium are sucrose 20 g, glucose 10 g, peptone 10 g, yeast extract 5 g, soybean 50 g, potato 100 g, in 1 L liquid medium.