Profiling serum antibodies to Mycobacterium tuberculosis
proteins in rhesus monkeys with nontuberculous Mycobacteria |
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Authors: | Fangui Min Jinchun Pan Ruike Wu Meiling Chen Huiwen Kuang Weibo Zhao |
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Institution: | 1)Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China;2)Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China |
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Abstract: | Recent evidence indicates that the prevalence of diseases caused by nontuberculous
mycobacteria (NTM) has been increasing in both human and animals. In this study, antibody
profiles of NTM in rhesus monkeys (Macaca mulatta) were determined and
compared with those of monkeys infected with Mycobacterium tuberculosis
complex (MTBC). Antibodies against 10 M. tuberculosis proteins, purified
protein derivative (PPD), and mammalian old tuberculin (MOT) were detected in 14 monkeys
naturally infected with NTM by indirect ELISA. Sera from 10 monkeys infected with MTBC and
10 healthy monkeys were set as controls. All antigens showed high serological reactivities
to MTBC infections and low reactivities in healthy monkeys. NTM infections showed strong
antibody responses to MOT and PPD; moderate antibody responses to 16kDa, U1, MPT64L,
14kDa, and TB16.3; and low antibody responses to 38kDa, Ag85b, CFP10, ESAT-6, and
CFP10-ESAT-6. According to the criteria of MTBC, only CFP10, ESAT-6, and CFP10-ESAT-6
showed negative antibody responses in all NTM infections. Taken together, these results
suggest that positive results of a PPD/MOT-based ELISA in combination with results of
antibodies to M. tuberculosis-specific antigens, such as CFP10 and
ESAT-6, could discriminate NTM and MTBC infections. Two positive results indicate an MTBC
infection, and a negative result for an M. tuberculosis-specific antigen
may preliminarily predict an NTM infection. |
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Keywords: | antibody M tuberculosis proteins nontuberculous mycobacteria rhesus monkeys |
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