Existence of atrial natriuretic polypeptide in kidney |
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| Authors: | M Sakamoto K Nakao M Kihara N Morii A Sugawara M Suda M Shimokura Y Kiso Y Yamori H Imura |
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| Affiliation: | 1. Second Division, Department of Medicine, Kyoto University School of Medicine, Kyoto, Japan;2. Radioisotope Research Center, Kyoto University, Kyoto, Japan;3. Department of Pathology, Shimane Medical University, Izumo, Japan;4. Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Kyoto, Japan;1. Centre hospitalo-universitaire de Lille, hôpital C-Huriez, service d’endocrinologie et métabolisme, 1, rue Polonovski, 59037 Lille cedex, France;2. UMR 1190 recherche translationnelle sur le diabète Inserm, 59000 Lille, France;3. EGID (European Genomic Institute for Diabetes), université de Lille, 59000 Lille, France;1. Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Japan;2. Department of Internal Medicine, Wakakusa-Tatsuma Rehabilitation Hospital, Japan;3. Department of Cardiovascular Medicine, Shinshu University School of Medicine, Japan |
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| Abstract: | Using a specific radioimmunoassay (RIA) for alpha-atrial natriuretic polypeptide (alpha-ANP), we have demonstrated the presence of alpha-rat ANP-like immunoreactivity (alpha-rANP-LI) in the rat kidney which is considered to be a target organ for atrial natriuretic polypeptides released from the heart. Most of alpha-rANP-LI was localized in the cortex. High performance gel permeation chromatography coupled with the RIA revealed that renal alpha-rANP-LI was eluted at the position of a low molecular weight form corresponding to alpha-rANP without detectable amounts of high molecular weight forms. This is in contrast to the observation that gamma-rANP, a high molecular weight form of 13k daltons, is the dominant form of alpha-rANP-LI in the rat atrium. In water-deprived rats, the concentration and content of alpha-rANP-LI in the kidney showed a significant decrease compared with control rats. In addition, the alpha-rANP-LI concentration and content in this organ revealed a substantial decrease after perfusion with physiological saline. These results indicate the existence of atrial natriuretic polypeptide (ANP) in the kidney and suggest that part of renal ANP may originate from the heart. |
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