Arabitol dehydrogenase as a selectable marker for rice |
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Authors: | P R LaFayette P M Kane B H Phan W A Parrott |
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Institution: | (1) Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Road, Athens, GA 30602-6810, USA;(2) Present address: Pioneer Hi-Bred International, 7100 NW 62nd Avenue, Johnston, IA 50131-1014, USA |
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Abstract: | Arabitol dehydrogenase has been adapted for use as a plant selectable marker. Arabitol is a five-carbon sugar alcohol that
can be used by E. coli strain C, but not by the laboratory K12 strains. The enzyme converts the non-plant-metabolizable sugar arabitol into xylulose,
which is metabolized by plant cells. Rice was transformed with a plant-expression-optimized synthetic gene using Biolistic-mediated
transformation. Selection on 2.75% arabitol and 0.25% sucrose yielded a transformation efficiency (9.3%) equal to that obtained
with hygromycin (9.2%). Molecular analyses showed that the atlD gene was integrated into the rice genome of selected plants and was inherited in a Mendelian manner. This study indicates
that arabitol could serve as an effective means of plant selection. |
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Keywords: | Transformation Non-antibiotic selection Positive selection Oryza sativa L |
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