Alterations in sugar residues in squamous metaplasia in hamster tracheal explants induced by benzo(a)pyrene and its reversal by retinoic ACID |
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Authors: | Dharam P Chopra Avarachan P Joiakim |
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Institution: | (1) Institute of Chemical Toxicology, Wayne State University, 2727 Second Avenue, Room 4000, 48201 Detroit, Michigan |
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Abstract: | Summary We have demonstrated that squamous metaplasia induced by benzo(a)pyrene (BP) in the hamster tracheal explants accompany distinct
alterations in carbohydrate moieties in the epithelial mucosa. Most prominent alterations were the preferential binding of
peanut agglutinin (PNA) and wheat germ agglutinin (WGA) in the basal cell layer in metaplastic lesions. In this study we examined
if reversal of BP-induced lesions by all-trans retinoic acid (RA) results in the acquisition of normal carbohydrate composition
by the tissue. Four lectins, PNA, WGA, Dolichos biflorus agglutinin, and Concanavalin A, in their horseradish peroxidase conjugates
were used. In control explants the intercellular plasma membrane of basal and mucous cells exhibited no significant reaction
with any of the lectins tested. In the metaplastic lesions induced by BP, PNA and WGA intensely stained the plasma membrane
and intercellular spaces of basal and intermediate cell layers; the granular layer cells did not bind PNA whereas they were
stained moderately with WGA. RA, which reversed the metaplasia, also conferred the tissue with lectin binding patterns similar
to that of control explants. These results thus show that the reversal of metaplasia is accompanied by acquisition of the
tissue’s original carbohydrate composition.
This research was supported by grant RO1-HL32308 from the National Heart, Lung and Blood Institute, Bethesda, MD. |
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Keywords: | vitamin A squamous metaplasia trachea lectins carbohydrates |
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