Determination of N-acetyl retigabine in dog plasma by LC/MS/MS following off-line microElution 96-well solid phase extraction |
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Authors: | Bu Wei Nguyen Mai Xu Christine Lin Chin-Chung Yeh Li-Tain Borges Virginia |
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Affiliation: | Valeant Research & Development, 3300 Hyland Avenue, Costa Mesa, CA 92626, USA. wbu@anacor.com |
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Abstract: | A high throughput off-line microElution 96-well solid phase extraction (SPE) followed by liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for the determination of N-acetyl retigabine in dog plasma has been developed and validated. The method involves the use of microElution 96-well SPE for the simultaneous extraction of N-acetyl retigabine and rapid removal of its N-glucuronide metabolite that has shown to be problematic due to its instability using other clean-up methods. The microElution SPE technology eliminates the need for post-extraction solvent evaporation and greatly reduces sample preparation time consequently improving assay efficiency. |
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