The reduction of the Rieske iron-sulfur cluster in naphthalene dioxygenase by X-rays |
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Authors: | Karlsson A Parales J V Parales R E Gibson D T Eklund H Ramaswamy S |
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Institution: | Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala. |
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Abstract: | Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO. |
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