High purification of the RNase inhibitor from rat liver by affinity chromatography |
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| Authors: | A A Gribnau J G Schoenmakers M van Kraaikamp H Bloemendal |
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| Affiliation: | 1. Center for Reproductive Medicine, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510120, Peoples Republic of China;2. Second School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510020, Peoples Republic of China |
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| Abstract: | A highly purified RNase inhibitor from rat liver can be obtained by chromatography on columns loaded with carboxymethyl cellulose-RNase. Polyacrylamide gel electrophoresis reveals that by this procedure a selective removal of non-inhibitor proteins has been achieved.As a consequence this factor is now available for the preparation of undegraded polyribosomes and ribosomal subunits. |
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