汉防己甲素及其联合氟康唑对白念珠菌细胞周期影响的初步研究

目的 探讨汉防己甲素联合氟康唑对白念珠菌细胞周期的影响.方法 将白念珠菌CA-1菌悬液与汉防己甲素和（或）氟康唑共培养12h,应用流式细胞仪测定空白对照组、汉防己甲素组、氟康唑组及汉防己甲素联合氟康唑组DNA含量.比较细胞周期各期DNA含量变化并计算增殖抑制率PI％,分析汉防己甲素及其联合氟康唑对白念珠菌细胞周期的影响.结果 汉防己甲素、氟康唑组与对照组S期DNA含量相比,分别能增加17.25％ （P =0.018）与6.54％ （P ＞0.05）,其联合运用效果更明显,S期DNA含量可增加31.52％ （P =0.002）.这说明汉防己甲素能将白念珠菌细胞阻滞在S期.结论 汉防己甲素能抑制白念珠菌细胞DNA合成,阻断白念珠菌细胞周期进程,抑制细胞分裂,其与氟康唑联用时阻滞作用更显著.

A preliminary research of tetrandrine and fluconazole affecting the cell cycle in Candida albicans

Objective To explore the effect of TET （tetrandrine） and fluconazole on the cell cycle in Candida albicans. Meth- otis Flow Cytometry was used to measure the DNA content of each cell cycle phase of the FLC （Fluconazole） sensitive strain CA-1, which was exposed to TET, FLC and TET ＋ FLC. The effect of TET （tetrandrine） and fluconazole on the cell cycle in Candida albicans was analysed by comparing DNA content of different cell cycle phases and calculating the PI% （ proliferation inhibition rate）. Results Compared with control group, TET and FLC could increase the DNA content of cell cycle in S-phase by 17.25% （ P = 0. 018） and 6.54% （ P 〉 0.05 ） respectively, while the combination use of TET and FLC could markedly increase S-phase DNA content by 3 I. 52% （ P = 0.002）. It showed that TET can arrest C. albicans ceils in S phase. Conclusions TET has potent effect of against Candida albieans by means of inhibiting DNA synthesis, arrest the cell cycle and inhibiting cell proliferation, while it would be more notable when combined with FLC.