Regulation of morphological and functional properties of astrocytes by hydrogen peroxide |
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Authors: | T. A. Kulahava G. N. Semenkova Z. B. Kvacheva S. N. Cherenkevich |
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Affiliation: | (1) Department of Biophysics, Physical Faculty, Belarussian State University, Minsk, 220050, Belarus;(2) Research Institute of Epidemiology and Microbiology, Republic of Belarus Ministry of Health, Minsk, 220050, Belarus |
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Abstract: | Effects of hydrogen peroxide on morphological characteristics, proliferation index, and menadione-dependent lucigenin-enhanced chemiluminescence of C6 glioma cells were studied. It was established that H2O2 at 5 × 10?7?1 × 10?8 M concentrations acted as a regulator of morphological and functional properties of astrocytes, inducing their reactivation, which is manifested as cell body hypertrophy and an increase of proliferative activity and menadione-induced production of superoxide anion radicals (O 2 ?? ). Cytodestructive action of hydrogen peroxide at a concentration higher than 1 × 10?6 M on C6 glioma cells shows itself as a decrease of their proliferation index and the ability to generate O 2 ?? under the effect of menadione. Use of lipopolysaccharide B as a functional stimulator has shown that H2O2 modifies signaling pathways leading to an increase of mitotic activity of C6 glioma cells and decreases the yield of lucigenin-dependent chemiluminescence of astrocytes under the action of menadione to the level of control values. |
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Keywords: | C6 glioma cells chemiluminescence hydrogen peroxide |
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