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Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor |
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| Authors: | H Jingami N Mizuno H Takahashi S Shibahara Y Furutani H Imura S Numa |
| Abstract: | DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion. |
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