Structural Analysis of rbcL Gene from an Endangered Plant，（Acanthopanax brachypus）

ＳｔｒｕｃｔｕｒａｌＡｎａｌｙｓｉｓｏｆｒｂｃＬＧｅｎｅｆｒｏｍａｎＥｎｄａｎｇｅｒｅｄＰｌａｎｔ，（Ａｃａｎｔｈｏｐａｎａｘｂｒａｃｈｙｐｕｓ）ＹＡＮＨｕａ－ｊｕｎ（严华军）；ＺＨＵＣｈｅｎｇ（朱）；ＷＵＮａｉ－ｈｕ（吴乃虎）（Ｉｎｓｔｉｔｕｔｅｏ...

Structural Analysis of rbcL Gene from an Endangered Plant,(Acanthopanax brachypus)

A 3.2 kb EcoRI DNA fragment containing the complete chloroplast rbcL gene from Acanthopanax brachypus has been cloned. In addition to the 1428 bp coding region encoding 475 amino acids of the gene, 278 bp upstream and 218 bp downstream were also sequenced. Possible ctpl and ctp2, equivalent to prokaryotic-35 and -10 elements, were found as TTGCGC and TACAAT respectively. The 5' untranslated leader region is 194 bp and the putative ribosome binding site in this region is GGAGG,located immediately upstream of the start codon. The 3' unrtanslated region contains two inverted repeat sequences, which in the mRNA might form stem-loop structures. The homology of rbcL amino acid sequence between A. brachypus and tobacco, spinach, pea, alfalfa, maize, rice, pine,Marchantia, Chlamydomonas and Anacystis are, respectively 93.05%,94.11%, 94.53%,89.68%, 92.21%, 2.21%, 92.63%, 87.58% and 80.84%.The promoter regions and part of the 5', 3' non-coding regions of rbcL from various plants were compared.