Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria |
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Authors: | L A R Sallam M E Osman A A Hamdy Gihan M Zaghlol |
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Institution: | (1) Microbial & Natural Products Chemistry Department, National Research Centre, Dokki, Cairo, Egypt;(2) Department of Botany & Microbiology, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt |
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Abstract: | Rice bran sample (12 Kg) was extracted and rice bran oil (RBO ≅ 76.8 g) was saponified. The resulted unsaponifiable matter
of RBO (RBO unsap) was qualitatively and quantitatively estimated using different chromatographic analyses. RBO, produced
9.65% unsaponifiable matter with the following contents, cholesterol, 6.75%; stigmasterol, 3.4%; β. sitosterol, 10.23% and
campesterol, 4.2%, in addition to unknown phytosterols, hydrocarbons and waxes. Microbial transformation process started by
screening of 35 bacterial strains, locally isolated from rice bran, air and soil, using RBO unsap as a carbon and an energy
source to produce some pharmaceutically useful C18 and C19 steroids. Moraxella ovis was the most potent isolate for its highest capability to utilize RBO unsap and selectively degrade the phytosterols side-chain
producing androst-4-ene-3,17-dione (AD), androsta-1,4-diene-3,17-dione (ADD), testosterone (T) and estrone (E). The RBO unsap
was the best carbon and energy source. Maximum production of the desired products was observed in 36 h, pH 7 and at 30°C by
M. ovis. |
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Keywords: | Androsta-1 4-diene-3 17-dione Androst-4-ene-3 17-dione Estrone Microbial transformation Moraxella ovis Phytosterols Rice bran oil Side-chain degradation Testosterone |
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