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Structure of a neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26 |
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| Authors: | Sánchez-Medina Inmaculada Gerwig Gerrit J Urshev Zoltan L Kamerling Johannis P |
| Affiliation: | Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, The Netherlands. |
| Abstract: | The neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26 in skimmed milk was found to be composed of d-glucose and d-galactose in a molar ratio of 2:3. Linkage analysis and 1D/2D NMR ((1)H and (13)C) studies performed on the native polysaccharide, and on an oligosaccharide obtained from a partial acid hydrolysate of the native polysaccharide, showed the polysaccharide to consist of branched pentasaccharide repeating units with the following structure. [structure: see text] |
| Keywords: | Lactobacillus delbrueckii spp. bulgaricus Exopolysaccharide Lactic acid bacteria Structural analysis NMR spectroscopy Mass spectrometry |
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