Somatic embryogenesis from immature zygotic embryos of Rosa bourboniana desp |
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Authors: | Navtej Kaur Pratap Kumar Pati Madhu Sharma P S Ahuja |
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Institution: | (1) Division of Biotechnology, Institute of Himalayan Bioresource Technology, 176 061 Palampur, HP, India;(2) Department of Botanical and Environmental Sciences, Guru Nanak Dev University, 143 005 Amritsar, India |
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Abstract: | Summary A protocol for the inducton of somatic embryogenesis from immature zygotic embryos of Rosa bourboniana, a scented rose species, was established. Somatic embryos were induced after 8wk of inoculation of zygotic embryos on MS
medium supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (5–15 μM). In addition to 2,4-dichlorophenoxy acetic acid concentrations, somatic embryogenesis was also influenced by the month of
collection of the explant and the stage of maturity of the hip. Maximum embryogenic response (16.6%) was observed using 2,4-dichlorophenoxy
acetic acid (15 μM), from green hips in the month of September. The use of l-proline (800 mg l−1) was found to be optimum for secondary embryogenesis. On periodic subculturing, the cultures formed somatic embryos sustainably
over a period of 18 mo. For somatic embryo germination, 6-benzylaminopurine (5 μM) was found to be most suitable. Rooted plants were transferred successfully to soil and appear morphologically normal under
greenhouse conditions. Transfer of plants for hardening was most suitable during the active growth period between June and
September.
IHBT Publication No: 0447 |
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Keywords: | 6-benzylaminopurine 2 4-dichlorophenoxyacetic acid histology plant growth regulators l-proline" target="_blank">l-proline scented rose |
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