Production of antibodies to roridin and their applications |
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Authors: | A A Burkin G P Kononenko V G Zoryan N A Soboleva E V Zotova |
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Institution: | (1) All-Russian Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, 123022 Moscow, Russia |
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Abstract: | Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate
allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues—roridin
A, verrucarin A, and verrucarol—amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine
roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 μg/kg. |
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