Asynchronous replication of p53 and 21q22 loci in chronic lymphocytic leukemia |
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Authors: | A Amiel Tali Litmanovich Elena Gaber Michael Lishner Lydia Avivi Moshe D Fejgin |
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Institution: | (1) Genetic Institute, Meir General Hospital, Kfar Saba, Israel 44281 Tel.: +972 9 7472540; Fax: +972 9 7419851, IL;(2) Department of Human Genetics, Tel Aviv University Medical School, Israel, IL;(3) Department of Medicine ‘A’, Meir Hospital, Kfar Saba, Israel, IL |
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Abstract: | In this study, in order to evaluate the replication pattern and the cell cycle dynamics of normal and malignant cells from
patients with chronic lymphocytic leukemia, we applied the FISH technique with the p53 gene. Asynchrony was determined by
the presence of one single and one set of double dots in the same cell. The rate of asynchronous replication was significantly
higher in malignant cells than in normal cells (a mean of 28 vs 13, respectively, P = 0.023). There were proportionately more cells with two single dots among the normal cells (P = 0.0047). These results probably reflect the changes in gene replication and cell cycle progression that occur in malignant
cells.
Received: 25 March 1997 / Accepted: 28 July 1997 |
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