青海藏族HLA II类基因多态性的研究

用PCR-RFLP的技术进一步研究了青海藏族HLA-DPB1的多态性。在19个HLA-DPB1 的等位基因中，共检出18个等位基因。其中，*0501的频率最高（AF=38.0%）；其次为*0201（AF=20.0%）；未检出*1601。在HLA-DPB1各等位基因的分布上，藏族与中国南方汉族、中国北方汉族等无明显差异，而与高加索人及尼格罗人的差异则较为显著。综合隶属于三大人种11个群体中的HLA-DQA1、-DQB1和-DPB1基因座各等位基因的分布频率，用UPGMA方法构建了分子系统树, 实验结果进一步证实汉藏同源说。Abstract:Following the study of the polymorphism of the HLA-DQA1and -DQB1in Tibetans of Qinghai Province, the polymorphism of the HLA-DPB1was investigated by the same technique―PCR-RFLP. Among the 19 detectable alleles of HLA-DPB1gene, 18 alleles were detected. The allele *0501 was the most frequent one (AF=38.0%); the allele *0201 (AF=20.0%）was the second one; and *1601 was not found by this technique. Compared with that of Southern Han and Northern Han nationality in China, the distribution of the alleles showed little difference; while compared with that of the Caucasoid and Negroid, it showed significant difference. The result of this report confirmed once more that the Tibetan and Han nationalities came from the same ancestors. Based on the allele frequencies of the three loci within HLAclass II region-HLA-DQA1、-DQB1and -DPB1from 11 groups among three main races in the world, a molecular phylogenetic tree was constructed with the method UPGMA.

Polymorphism of HLAClassII Genes in the Zang(Tibetan)Nationality (continued)