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Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set
Authors:Sugiura  Ko  Tomita  Sei  Minamoto  Toshifumi  Mishina  Tappei  Iwata  Akihisa  Abe  Tsukasa  Yamamoto  Satoshi  Watanabe  Katsutoshi
Affiliation:1.Division of Biological Sciences, Department of Zoology, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo, Kyoto, 606-8502, Japan
;2.Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada, Kobe, 657-8501, Japan
;3.Division of Southeast Asian Area Studies, Graduate School of Asian and African Area Studies, Kyoto University, 46 Yoshida-Shimoadachicho, Sakyo, Kyoto, 606-8501, Japan
;4.Biodiversity Research Division, LAGO Co., Ltd, 396-2 Taga, Omihachiman, Shiga, 523-0821, Japan
;
Abstract:Ichthyological Research - We report a novel species-specific primer set for detection of the endangered botiid loach Parabotia curtus in environmental waters. A primer test using DNA extracted from...
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