| 蓼属头状蓼组rDNA-ITS的序列扩增及分析 |
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| 引用本文： | 向红,孙爱群,翁贵英,杨友联,王绪英.蓼属头状蓼组rDNA-ITS的序列扩增及分析[J].西北植物学报,2010,30(5). |
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| 作者姓名： | 向红 孙爱群 翁贵英 杨友联 王绪英 |
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| 作者单位： | 六盘水师范学院,贵州六盘水,553004 |
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| 基金项目： | 六盘水师范学院科研基金项目 |
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| 摘 要： | 以贵州境内蓼属头状蓼组6种(含1变种)植物为材料,对其rDNA的内转录间隔区(ITS)序列进行PCR扩增,得到6种植物的ITS序列,分别为:赤胫散2个居群(Polygonum runcinatum var.sinense,GenBank登录号FJ606887、FJ648802),平卧蓼(P.strindbergii,GenBank登录号FJ648803 ),尼泊尔蓼(P.nepalense,GenBank登录号FJ648804),羽叶蓼(P.runcinatum,GenBank登录号FJ648805),火炭母(P.chinense,GenBank登录号FJ648806)和头花蓼(P.capitatum,GenBank登录号FJ648807).其中赤胫散与平卧蓼的ITS序列为首次报道.序列分析结果表明,蓼属头状蓼组6种植物ITS序列总长度为661～666 bp,ITS1区序列长度为243～246 bp,5.8 S rDNA区序列长度165 bp,ITS2区序列长度253～258 bp,6种植物的差异主要集中在ITS1和ITS2区.聚类分析显示,6种头状蓼组植物具有共同起源,结果支持赤胫散从羽叶蓼变种上升为独立物种.
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| 关 键 词： | 蓼属 内转录间隔区(ITS)序列 系统分析 |
Sequence Analysis of Internal Transcribed Spacer of Polygonum Sect.Cephalophilon and Its Systematic Utility |
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| XIANG Hong,SUN Ai-qun,WENG Gui-ying,YANG You-lian,WANG Xu-ying.Sequence Analysis of Internal Transcribed Spacer of Polygonum Sect.Cephalophilon and Its Systematic Utility[J].Acta Botanica Boreali-Occidentalia Sinica,2010,30(5). |
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| Authors: | XIANG Hong SUN Ai-qun WENG Gui-ying YANG You-lian WANG Xu-ying |
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| Institution: | XIANG Hong,SUN Ai-qun,WENG Gui-ying,YANG You-lian,WANG Xu-ying(Liupanshui Normal College,Liupanshui,Guizhou 553004,China) |
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| Abstract: | With 6 species(including 1 variety)of Polygonum Sect.Cephalophilon which distributed in Guizhou Province as experimental material,the sequences of rDNA internal transcribed spacer(ITS) were amplified by means of PCR.All 7 sequences of 6 species of Polygonum Sect.Cephalophilon were deposited in GenBank,there are 5 species and 1 variations populations based on morphological character in the district,Polygonum runcinatum var.sinense(Genbank accession number FJ606887,FJ648802),P.strindbergii(Genbank accession n... |
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| Keywords: | Polygonum internal transcribed spacer(ITS) phylogenetic analysis |
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