A colorimetric method for the determination of lipase activity in soil |
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| Authors: | R. Margesin G. Feller M. Hämmerle U. Stegner F. Schinner |
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| Affiliation: | (1) Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria;(2) Laboratory of Biochemistry, Institute of Chemistry, University of Liège, B-4000 Liège, Belgium |
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| Abstract: | A colorimetric method for the determination of lipase activity in soil has been developed. Using p-nitrophenyl butyrate as substrate, soil samples are incubated at 30 °C and pH 7.25 for 10 min. After cooling on ice and centrifugation, the released p-nitrophenol is determined at 400 nm. To allow for the adsorption of p-nitrophenol onto soil, a calibration curve is prepared in the presence of soil. |
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| Keywords: | lipase activity mineral-oil-contamination p-nitrophenyl butyrate soil |
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