Occurrence of immunoreactivity for adipocyte-type fatty acid binding protein in degenerating granulosa cells in atretic antral follicles of mouse ovary |
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Authors: | Mohammad Reza Nourani Yuji Owada Noriko Kitanaka Hiroyuki Sakagami Hisae Hoshi Hiroo Iwasa Friedrich Spener Hisatake Kondo |
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Affiliation: | (1) Division of Histology, Department of Cell Biology, Graduate School of Medicine, Tohoku University, Sendai, Japan;(2) Department of Biochemistry, University of Meuenster, Muenster, Germany;(3) Department of Anatomical Sciences, Baghiyatollah Medical Sciences University, Teheran, Iran |
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Abstract: | The localization of adipocyte-type fatty acid binding protein (A-FABP) in the mature mouse ovary was examined by immuno-light and electron microscopy. Solitary round cells showing the distinct immunoreactivity for A-FABP were detected in 1–6 antral follicles. In sets of two consecutive sections in a mirror alignment on slide glasses which were treated for immunoreactivity for A-FABP and TUNEL reaction separately, cells immunoreactive for A-FABP appeared in the same antral follicles as containing cells exhibiting TUNEL-reaction. In immunoelectron microscopy, A-FABP-immunopositive cells were found to contain highly electron-dense nuclei of round, irregular or crescent shapes together with cytoplasmic remnants without any features of macrophages or cells of extrinsic origin. Therefore the cells were identified as apoptotic granulosa cells. The apoptotic cells immunoreactive for A-FABP were often seen to be enclosed/engulfed in adjacent cells exhibiting normal ultrastructures without containing numerous lysosomes. The present findings suggest that A-FABP is involved in the apoptosis of ovarian granulosa cells, probably through its interaction with peroxisome proliferator activated receptors. |
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Keywords: | A-FABP Cell degeneration Granulosa cell Ovary Mouse |
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