Effectiveness of TaqMan probes for detection of fish eggs and larvae in the stomach contents of a teleost predator |
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Authors: | Hunter E Taylor N Fox C J Maillard M Taylor M I |
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Affiliation: | Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk NR33 OHT, U.K. ewan.hunter@cefas.co.uk |
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Abstract: | Experiments were conducted on the ability of TaqMan molecular probes to detect plaice Pleuronectes platessa DNA from eggs, and cod Gadus morhua DNA from eggs and larvae following ingestion by a teleost predator, whiting Merlangius merlangus. Estimated half-life detection rate (T50) for eggs was 31 h, and 26 h for larvae, with some positive detections occurring even after visual inspection indicated complete gut clearance. Because TaqMan probes are taxon specific, the results presented demonstrate that this technique can provide a means of rapid and unambiguous detection of predation by teleosts on fish eggs and larvae. |
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Keywords: | digestion experiments Gadus morhua Merlangius merlangus Pleuronectes platessa stomach contents TaqMan real‐time PCR assay |
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