A subnanosecond-pulse fluorometric study of the CA2+ and MG2+ induced conformational changes on S-100a protein |
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Authors: | L Brox D Hunting A Belch |
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Institution: | McEachern Laboratory and Cross Cancer Institute, University of Alberta, Edmonton, Alberta, Canada T6G 2H7 |
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Abstract: | The fluorescence parameters of the single tryptophan residue (Trp 90 alpha) in S-100a (alpha beta) protein have been studied by steady state fluorimetry and by subnanosecond fluorescence decays excited by pulsed-picosecond laser system. At pHs 7.1 and 8.5, double exponential decays were consistently observed. At both pHs, Ca2+ and to a less extent Mg2+ ions proved to modify the percentage contribution of the two decaying species. The interest of the finding is discussed. |
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Keywords: | Author to whom reprint requests should be directed |
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