| 香菇多糖产生菌的原生质体诱变选育 |
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| 引用本文： | 陈琼华,张志光,张天晓.香菇多糖产生菌的原生质体诱变选育[J].激光生物学报,2001,10(4):288-292. |
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| 作者姓名： | 陈琼华 张志光 张天晓 |
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| 作者单位： | 1. 广州大学生物与化学工程学院,
2. 湖南师范大学生命科学学院, |
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| 基金项目： | 湖南省科委自然科学基金资助项目 (97JKY2 0 0 4- 8) |
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| 摘 要： | 对香菇原生质体进行不同时间紫外线照射诱变，实验结果表明：采用紫外线照射120S，可获得高产突变株，该突变株BM－43的多糖产量由出发菌株B15的235mg/100ml提高到278.75mg/100ml，产多糖能力提高了18.62%，经连续传代3次，产多糖能力仍保持稳定。说明原生质体诱变5技术用于大型真菌多糖高产菌株的选育是一条行之有效的途径。
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| 关 键 词： | 香菇多糖 原生质体 诱变 选育 高产菌株 |
| 文章编号： | 1007-7146(2001)04-0288-05 |
| 修稿时间： | 2001年4月12日 |
Strain Improvement of Polysaccharide-producing of the lentinus Edodes by Protoplast Mutagenesis |
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| CHEN Qiong-hua,ZHANG Zhi-guang,ZHANG Tian-xiao.Strain Improvement of Polysaccharide-producing of the lentinus Edodes by Protoplast Mutagenesis[J].ACTA Laser Biology Sinica,2001,10(4):288-292. |
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| Authors: | CHEN Qiong-hua ZHANG Zhi-guang ZHANG Tian-xiao |
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| Institution: | CHEN Qiong-hua1,ZHANG Zhi-guang2,ZHANG Tian-xiao2 |
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| Abstract: | the protoplast-UV mutagenesis for increasing polysaccharide was studied on lentinus edodes strain. The experiments showed that the high mutant could by obtained by UV-irradiation of the protoplasts for 120 seconds. The polysaccharide content of the mutant BM-43 was increased by 18.62% than that of the starting strain B15. The results in dicated that the protoplast mutagenesis was a better approach to incresas polysaccharide yield of Lentinus edodes. |
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| Keywords: | Lentinus edodes Protoplast Mutagenesis Polysaccharide |
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