Prognostic value of assays for circulating immune complexes and natural cytotoxicity in malignant skin melanoma (stages I and II) |
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| Authors: | E Kristensen I Brandslund H Nielsen S -E Svehag |
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| Institution: | (1) Fibiger Laboratory, Copenhagen, Denmark;(2) Institute of Medical Microbiology, Odense University, Denmark;(3) Present address: Medical-gastroenterological Department C, Herlev University Hospital, Herlev Ringvej, DK-2730 Herlev, Denmark |
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| Abstract: | Summary The aim of the present study was to determine the possible prognostic value of immune complex determinations and estimations of natural cytotoxicity in melanoma patients. Circulating immune complexes were assayed in 46 patients suffering from malignant skin melanoma (stages I and II) by a direct complement consumption (CC) test, a polyethylene glycol (PEG) precipitation CC assay and a solid-phase Clq-protein A-binding assay. The presence of both IgG- and IgM-containing complexes in IC-positive melanoma sera was confirmed by an Ig-class-specific precipitation-immunoradiometric assay. Results based on this assay and reduction-alkylation data indicated that IgM-containing IC were responsible for a major part of the IC activity recorded in the CC-assays.Soluble immune complex activity, as measured by the direct CC test and the PEG-CC assay, correlated with relapse, whereas the expected inverse relationship between natural cytotoxicity and recurrence was not established. This finding may be explained by the apparent predominance of IgM-containing immune complexes in patients subsequently showing relapse. |
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