Activation of Dopamine Receptors Does Not Affect Phosphoinositide Turnover in NCB-20 Cells |
| |
Authors: | A. B. Cubitt B. L. Brown P. R. M. Dobson |
| |
Affiliation: | Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Sheffield, England |
| |
Abstract: | Dopamine inhibits and serotonin stimulates adenylate cyclase activity in a neuroblastoma X Chinese hamster brain explant cell line (NCB-20). The inhibition of cyclic AMP accumulation by dopamine was blocked by pretreatment of the cells with pertussis toxin. Carbachol and bradykinin stimulated the accumulation of water-soluble inositol phosphates whereas thyrotropin-releasing hormone, vasopressin, neurotensin, and phenylephrine were without effect. Dopamine and serotonin had no significant effect on carbachol-induced phosphoinositide hydrolysis or the levels of the parent lipids within the membrane. Forskolin induced a much larger stimulation of cyclic AMP than did serotonin, and caused an increase in the levels of phosphatidylinositol-4-phosphate and phosphatidyl inositol-4,5-bisphosphate in the cell membrane. |
| |
Keywords: | Dopamine Serotonin Cyclic AMP Phosphoinositide turnover Neuroblastoma |
|
|