Identification of a Lysine Residue Important for the Catalytic Activity of Yeast Farnesyl Diphosphate Synthase |
| |
Authors: | Marc J C Fischer Sophie Meyer Patricia Claudel Marc Bergdoll Francis Karst |
| |
Institution: | 1.Laboratoire Métabolisme Secondaire de la Vigne,Univ. Strasbourg, INRA, Inst. Natl. Recherche Agron.,Colmar,France;2.The Inst. Biol. Mol. Plantes,Univ Strasbourg, CNRS,Strasbourg Cedex,France |
| |
Abstract: | The Saccharomyces cerevisiae
ERG20 gene (encoding farnesyl diphosphate synthase) has been subjected to a set of mutations at the catalytic site, at position
K254 to determine the in vivo impact. The mutated strains have been shown to exhibit various growth rates, sterol profiles
and monoterpenol producing capacities. The results obtained suggest that K at position 254 helps to stabilize one of the three
Mg2+ forming a bridge between the enzyme and DMAPP, and demonstrate that destabilizing two of the three Mg2+ ions, by introducing a double mutation at positions K197 and K254, results in a loss of FPPS activity and a lethal phenotype. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|