提高转基因表达策略研究进展

随着转基因技术在各个应用领域的深入和发展 ,转基因沉默现象已引起越来越多人的关注。在介绍转基因沉默的概念、分子机理的基础上 ,着重对提高转基因表达的方法、策略及其研究进展进行综述。分别阐述内含子、组织特异性启动子、增强子、定点整合、DNA大片段、反式作用因子以及基因组调控元件核基质附着区对转基因表达的影响。为克服转基因失活、提高转基因的表达效率提供理论依据。

Advances About the Strategy of Improving the Transgene Expression

With the technique of transgene having been applied to every fields, the phenomenon of transgene silencing has beening attracted more and more attentions. This paper emphasized to summarize the methods, the policies and the progresses of improving transgene expression based on investigating about the concept and molecule mechanism of transgene silencing. Next factors, such as the intron, the tissue special promotor and enhancer, the targeting integration, the DNA big fragments, the trans acting elements, the matrix association regions(MARs), which affect transgene exprssion had been expatiated respectively. It will provide the theory bases which overcome transgene silencing and improve the expression efficiency of transgene.