Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme. |
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| Authors: | T Nesbakken P Kolsaker and J Ormerod |
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| Institution: | Department of Biology, University of Oslo, Norway. |
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| Abstract: | The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate. |
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