Influence of osmotica and abscisic acid on triglyceride accumulation in peanut somatic embryos |
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Authors: | V B Mhaske K Chengalrayan S Hazra |
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Institution: | (1) Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, India Fax no: +91-212-349038 E-mail: shazra@dalton.ncl.res.in, IN |
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Abstract: | Attempts were made to determine the influence of sucrose, mannitol, sorbitol and abscisic acid on accumulation of triglycerides
in peanut (Arachis hypogaea L.) somatic embryos. The results revealed that 0.584 m sucrose in the medium produced increased triglycerides in the embryos compared to the control. At 0.730 m sucrose, embryos were necrotic although the triglyceride content was high. Sorbitol at 0.6 m or abscisic acid at 20 μm were effective in increasing triglycerides in the embryos. The increase in triglycerides on a fresh-weight basis observed
with increasing concentration of osmoticium was not apparent when determined in terms of dry weight. However, an increase
in triglyceride as percent fresh weight observed in the presence of 20 μm abscisic acid remained unaltered when determined in terms of percent dry weight. An increase in storage lipid did not improve
conversion of peanut somatic embryos.
Received: 4 April 1997 / Revision received: 15 February 1998 / Accepted: 2 March 1998 |
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Keywords: | Sucrose Mannitol Sorbitol Osmolality Abscisic acid |
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