| 抗家蚕浓核症病毒单克隆抗体的制备及其在诊断上的应用 |
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| 引用本文： | 陈建国 马可. 抗家蚕浓核症病毒单克隆抗体的制备及其在诊断上的应用[J]. 病毒学报, 1989, 5(1): 77-82 |
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| 作者姓名： | 陈建国 马可 |
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| 作者单位： | 浙江农业大学 杭州(陈建国，滕俊琳，胡萃)，马里兰大学 美国(马可) |
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| 摘 要： |
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| 关 键 词： | 蚕浓核症病毒 单克隆抗体 抗体制备 |
PRODUCTION OF MONOCLONAL ANTIBODIES TO DENSOVIRUS OF SILKWORM ( BOMBYX MORI ) AND THEIR APPLICATION IN DIAGNOSIS |
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| Chen Jianguo Teng Junlin Hu Cui Michael Ma Zhejiang Agricultural University,Hangzhou,ChinaUniversity of Maryland,College Park,Md. ,U.S.A.. PRODUCTION OF MONOCLONAL ANTIBODIES TO DENSOVIRUS OF SILKWORM ( BOMBYX MORI ) AND THEIR APPLICATION IN DIAGNOSIS[J]. Chinese journal of virology, 1989, 5(1): 77-82 |
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| Authors: | Chen Jianguo Teng Junlin Hu Cui Michael Ma Zhejiang Agricultural University Hangzhou ChinaUniversity of Maryland College Park Md. U.S.A. |
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| Affiliation: | Chen Jianguo Teng Junlin Hu Cui Michael Ma Zhejiang Agricultural University,Hangzhou,ChinaUniversity of Maryland,College Park,Md. 20742,U.S.A. |
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| Abstract: | Monoclonal antibodies ( McAbs ) to densovirus ( DNV ) of silkworm ( Bombyx mori ) were generated by PEG fusion of splenocytes from immunized Balb/c mice and FO myeloma cells. After extensive screening, 6 hybridoma cell lines ( BmD1-6 ) were selected.IgG8 were secreted by BmD1,2,5, and IgG24, by BmD3, 4,6. The highest titer of ascites induced by these hybridoma cell lines was around 256 wfth double immunodifusion test and around 250 000 with ELISA indirect method. All McAbs secreted by these hybridoma cell lines can effectively neutralize the infectivity of BmDNV to silkworm. The highest neutralization index was around 1 096.McAbs Dot-ELISA direct, indirect, and sandwich methods were developed for the detection of BmDNV antigen from silkworm. The sensitivity of Dot-ELISA indirect method was 1 024 times that of double immunodifusion test, and 256 times that of countercurrect immunoelectrophoresis. The results demonstrated that these methods were specific, sensitive, fast and simple and may be used for the diagnosis and epidemiological studies of BmDNV |
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| Keywords: | Densovirus of silkworm Monoclonal antibodies Dot-ELISA |
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