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Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells |
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| Authors: | Islam Mohammed Omedul Kanemura Yonehiro Tajria Jesmin Mori Hideki Kobayashi Satoshi Shofuda Tomoko Miyake Jun Hara Masayuki Yamasaki Mami Okano Hideyuki |
| Institution: | Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Hyogo 661-0974, Japan. |
| Abstract: | We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker. |
| Keywords: | ABC transporter ATP-binding cassette transporter MDR multidrug resistance P-gp P-glycoprotein hNSPCs human neural stem/progenitor cells SP side-population Rh123 rhodamine 123 |
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