Detection of nitric oxide in tissue by spin trapping EPR spectroscopy and triacetylglycerol extraction |
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| Authors: | Guangyin Zhou Baolu Zhao Jingwu Hou Meifen Li Chang Chen Wenjuan Xin |
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| Affiliation: | (1) Institute of Biophysics, Academia Sinica, 15 Datun Road, Chaoyang District, Beijing, 100101, China |
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| Abstract: | A modified method based on EPR spin trapping and triacetylglycerol extraction was used for tissue nitric oxide (NO) detection at room temperature. NO signal intensity was stable for about 1.5 h and the detection limit of this method was less than 200 pmol g–1 tissue. Using this method, we report evidence that NO production in vivo can be inhibited by adriamycin in mice livers. |
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| Keywords: | adriamycin EPR nitric oxide spin trapping triacetylglycerol |
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