Multiple forms of amylase in leaf extracts: electrophoretic transfer of the enzyme forms into amylose-containing polyacrylamide gels |
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Authors: | M Steup K P Gerbling |
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Affiliation: | Laboratoire de Physiologie cellulaire végétale, CNRS-INRA, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France |
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Abstract: | A method for the analysis of multiple forms of glucan-degrading enzymes is described. The procedure consists of the separation of the proteins by electrophoresis or isoelectric focusing in glucan-free polyacrylamide gels followed by the nondenaturing electrophoretic transfer into a second polyacrylamide layer which contains immobilized glucans. The method combines the resolving power of electrophoretic separations in glucan-free media with the sensitivity of amylase activity detection in amylose-containing polyacrylamide gels. The procedure is especially useful when samples containing low amylase activity, but a large number of multiple enzyme forms, are to be analyzed. |
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Keywords: | plant cells vacuoles To whom correspondence should be addressed. |
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