Characterization of Phages Virulent for <Emphasis Type="Italic">Robinia pseudoacacia</Emphasis> Rhizobia |
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Authors: | Wanda Ma?ek Sylwia Wdowiak-Wróbel Micha? Bartosik Gra?yna Konopa Magdalena Narajczyk |
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Institution: | (1) Department of Genetics and Microbiology, M. Curie-Skłodowska University, Akademicka 19 Street, Lublin, 20-033, Poland;(2) Department of Molecular Biology, University of Gdańsk, Kładki 24 Street, Gdańsk, 80-822, Poland |
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Abstract: | Three lytic phages (ΦRP1, ΦRP2, and ΦRP3) specific for Robinia pseudoacacia rhizobia were isolated from the soil under black locust. They were characterized by their morphology, host range, and some
other properties including DNA molecular weights. Studied phages have been found to belong to Siphoviridae family that comprises viruses with long, and noncontractile tails. They had broad host ranges and effectively lysed not only
Robinia pseudoacacia microsymbionts but also different Mesorhizobium species. The phages were homogenous in latent periods (300 min) but heterogeneous in burst sizes (100–200 phage particles
per one infected cell) and rise periods (90–120 min). They showed a distinct adsorption rate to Robinia pseudoacacia rhizobia (70.4–93.94%). The molecular weights of phage DNAs estimated from restriction enzyme digests were in the range from
ca. 82 kb to ca. 105 kb. |
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