DNA methylation as a key process in regulation of organogenic totipotency and plant neoplastic progression? |
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Authors: | Pascal Lambé Hity Schié Nkung Mutambel Jean-Gabriel Fouché Roger Deltour Jean-Michel Foidart Thomas Gaspar |
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Institution: | (1) Laboratoire de Morphologie, Institut de Biologie Végétale B22, Sart Tilman, B-4000 Liège, Belgium;(2) Laboratoire d’Hormonologie Fondamentale et Appliquée, Institut de Biologie Végétale B22, Sart Tilman, B-4000 Liège, Belgium;(3) Laboratoire de Biologie Générale, Institut de Pathologie B 23, CHU, Sart Tilman, B-4000 Liège, Belgium |
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Abstract: | Summary Progressive loss of organogenic totipotency appears to be a common event in long-term plant tissue culture. This loss of totipotency,
which has been proposed to be a typical trait of plant neoplastic progression, is compared to some mechanisms that occur during
the establishment of animal differentiation-resistant cancer lines in vitro. Evidence is presented that alteration in DNA methylation patterns and expression of genes occur during long-term callus
culture. An effect of the auxin, 2,4-dichlorophenoxyacetic acid, in the progressive methylation, is moreover suggested. Methylation
of genes relevant to cell differentiation and progressive elimination of cells capable of differentiation is proposed as being
responsible for this progressive loss of organogenic potential. Finally, the epigenetic alteration (DNA methylation) that
occurs during prolonged periods of culture may induce other irreversible genetic alterations that ultimately make the loss
of totipotency irreversible. |
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Keywords: | in vitro cultures totipotency habituation neoplastic progression DNA methylation 2 4-D |
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