Selective loss of <Emphasis Type="Italic">lin</Emphasis> genes from hexachlorocyclohexane-degrading <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> ITRC-5 under different growth conditions |
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Authors: | Atul K Singh Pankaj Chaudhary Ankit S Macwan Upendra N Diwedi Ashwani Kumar |
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Institution: | (1) Environmental Biotechnology Section, Industrial Toxicology Research Center, Mahatma Gandhi Marg, Lucknow, 226001, India;(2) Department of Biochemistry, University of Lucknow, Lucknow, 226007, India |
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Abstract: | The chlorinated insecticide γ-hexachlorocyclohexane (γ-HCH) is sequentially metabolized by the products of linA, linB, linC, linD, linE, and linF genes to β-ketoadipate, which is subsequently mineralized. Two or more copies of these genes are present in the bacterium
Pseudomonas aeruginosa ITRC-5 that was isolated earlier by selective enrichment on technical-HCH. At least one copy of linA, linB, linC, linD, and possibly linE is lost from ITRC-5 upon its growth on γ-HCH. All the lin genes, however, are lost when the bacterium was grown in Luria–Bertani (LB) medium. The loss of lin genes is accompanied with the loss/rearrangement of insertion sequence IS6100 genes. Concomitant to the loss of lin genes, the degradation of HCH-isomers by “γ-HCH grown cells” is slower, when compared with “technical-HCH grown cells”, and
is completely lost by “LB-grown cells”. The selective loss of lin genes during different growth conditions has not been reported before and is expected to help in understanding the dynamism
of degradative genes. |
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Keywords: | Hexachlorocyclohexane Biodegradation lin genes IS6100 Pseudomonas aeruginosa |
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