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PBAD-Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in Pseudomonas and Burkholderia spp. and Other Bacteria |
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| Authors: | Dongru Qiu F. Heath Damron Takehiko Mima Herbert P. Schweizer Hongwei D. Yu |
| Abstract: | We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads. |
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