| SMAD4在嗜甲醇酵母中的表达及鉴定 |
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| 引用本文： | 刘一平,黄翠芬.SMAD4在嗜甲醇酵母中的表达及鉴定[J].生物化学与生物物理学报,2000,32(3):239-242. |
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| 作者姓名： | 刘一平 黄翠芬 |
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| 作者单位： | 广州军区医学研究所!广州510507(刘一平)，军事医学科学院生物工程研究所!北京100071(杨晓，邓继先，程萱，黄翠芬)，美国国立卫生研究院糖尿病、消化道病和肾病研究所!贝塞斯达MD20892(徐晓玲) |
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| 摘 要： | Ｓｍａｄ４是新近发现的一种肿瘤抑制基因。在５０％的胰腺癌和３０％的结肠癌中，此基因发生突变或缺失。利用Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ酵母表达系统，以胞外分泌的方法表达了ＳＭＡＤ４蛋白，并对ＳＭＡＤ４蛋白的生化性质进行了分析。结果显示表达蛋白的分子量约６７ｋＤ，经Ｗｅｓｔｅｒｎ印迹鉴定，与ＳＭＡＤ４抗全有特异的结合反应，Ｎ末端１３个氨基酸的序列与Ｓｍａｄ４ ｃＤＮＡ推断的序列完全一致。
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| 关 键 词： | Smad4 嗜甲醇酵母表达系统 鉴定 肿瘤抑制基因 |
Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein |
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| LIU Yi Ping ,YANG Xiao,XU Xiao Ling ,DENG Ji Xian,CHENG Xuan,HUANG Cui Fen.Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein[J].Acta Biochimica et Biophysica Sinica,2000,32(3):239-242. |
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| Authors: | LIU Yi Ping YANG Xiao XU Xiao Ling DENG Ji Xian CHENG Xuan HUANG Cui Fen |
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| Institution: | LIU Yi Ping 1,YANG Xiao,XU Xiao Ling 2,DENG Ji Xian,CHENG Xuan,HUANG Cui Fen * |
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| Abstract: | Smad 4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad 4 cDNA, and it was able to specifically react with the antibody against SMAD4. |
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| Keywords: | Smad 4 Pichia pastoris yeast expression |
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